TECHNOLOGY | pHion Therapeutics

Phion technology

pH Responsive

Cationic

Helical

Conditional Cell Penetration

RALA is a 30 amino acid peptide with the following advantages for precision drug delivery:

Secondary

Amphipathic

RALA nanoparticles have many advantages

High cell transfection / cellular entry without associated toxicity

Self-assembly with nucleic cargo, irrespective of size

Stable nanoparticle with no need for cold chain storage

Non-immunogenic nanoparticles facilitate repeated dosing

Protection of drug cargo while increasing circulatory half-life

Demonstrated formulation lyophilisation and easy scale up

RALA APPLICATIONS

RALA is optimised for both localised and systemic delivery, excelling in in vitro, in vivo and ex vivo applications.

RALA-V is an optimised RALA & triple-plasmid nanoparticle system for efficient and cost-effective production of AAV viral vectors for use in gene therapy applications

RALA

RALA-V

PUBLICATIONS

Over 20+ studies have been published using the RALA system, ranging from the fundamental design and application of an efficient cell penetrating peptide to use as a potential therapeutic mRNA vaccine candidate.

Below is a selection of publications of special note:

McCarthy HO et al. Development and characterization of self-assembling nanoparticles using a bio-inspired amphipathic peptide for gene delivery.

J Control Release. 2014 Sep 10;189:141-9.

Udhayakumar VK et al. Arginine-Rich Peptide-Based mRNA Nanocomplexes Efficiently Instigate Cytotoxic T Cell Immunity Dependent on the Amphipathic Organization of the Peptide.

Adv Healthc Mater. 2017 Jul;6(13).

Ali AA et al. DNA vaccination for cervical cancer; a novel technology platform of RALA mediated gene delivery via polymeric microneedles.

Nanomedicine. 2017 Apr;13(3):921-932.

McCrudden CM et al. Systemic RALA/iNOS nanoparticles; a potent gene therapy for metastatic breast cancer coupled as a biomarker of treatment.

Mol Ther Nucleic Acids. 2017 Mar 17;6:249-258.

Cole G et al. Dissolving microneedles for DNA vaccination: improving functionality via polymer characterisation and RALA complexation.

Hum Vaccin Immunother. 2017 Jan 2;13(1):50-6.

Cole G et al. DNA vaccination for cervical cancer: Strategic optimisation of RALA mediated gene delivery from a biodegradable microneedle system.

Eur J Pharm Biopharm. 2018 Jun;127:288-297.

Cole G et al. DNA vaccination via RALA nanoparticles in a microneedle delivery system induces a potent immune response against the endogenous prostate cancer stem cell antigen.

PHION TECHNOLOGY